Influence of disinfectants on growth of biofilm formed by <i>К. pneumoniae</i> strains

Introduction . Currently, the problem of biofilm-associated infections is great importance, since classical methods antibiotic therapy for purulent-inflammatory diseases become ineffective due to high resistance pathogens. An urgent task find ways suppress adhesion processes or destroy an already formed biofilm using various disinfectants, enzymes, a combination antibacterial and other drugs. Materials The object study bacteria Klebsiella pneumoniae isolated from wounds fistulas patients with chronic osteomyelitis. Biofilms were cultured two days in wells polystyrene plates on coverslips. In control series experiment, biofilm-forming ability K. strains was studied after 48 hours. second series, 24 hours, emerging exposed solution 3 % hydrogen peroxide, third skin antiseptic, intensity formation assessed. For statistical processing obtained data, Gnumeric 1.12.17 spreadsheet program used. Results its discussion had ability. impact disinfectants led decrease optical density formation. On cover slips total proportion microcolonies individual adherent cells observed. Disinfectants (hydrogen peroxide antiseptic) bacteriostatic effect biofilms by strains. addition, bactericidal antiseptic planktonic bacterial found. Conclusions data must be taken into account selection effective used clinic prevent emergence resistant microbes.